Using a Microscope
- Place the microscope on a table gently, with the arm facing you.
- Look through the eyepiece and adjust the mirror so that light is reflected through the hole in the stage.
- Place the slide on the stage and secure it with the clip hole.
- Adjust the diaphragm so that the greatest amount of light pass through the opening (the circle of light that you see is called the field of view).
- Turn the nosepiece so that the low power objective (10x) clicks into place.
- Focus the low power objective lens by turning the coarse adjustment knob.
- Turn the nosepiece again until the high power objective lens clicks into place.
- Focus the high power objective lens by turning the fine adjustment knob.
- Always click the low power objective lens back into place over the field of view when you finish using the microscope.
Note: Usually the least magnification used in order to be able to see something in the water sample is 400x – so you need to use the 10x eyepiece and 40x objective lens.