Using A Microscope

5 01 2012

 

Using a Microscope

  1. Place the microscope on a table gently, with the arm facing you.
  2. Look through the eyepiece and adjust the mirror so that light is reflected through the hole in the stage.
  3. Place the slide on the stage and secure it with the clip hole.
  4. Adjust the diaphragm so that the greatest amount of light pass through the opening (the circle of light that you see is called the field of view).
  5. Turn the nosepiece so that the low power objective (10x) clicks into place.
  6. Focus the low power objective lens by turning the coarse adjustment knob.
  7. Turn the nosepiece again until the high power objective lens clicks into place.
  8. Focus the high power objective lens by turning the fine adjustment knob.
  9. Always click the low power objective lens back into place over the field of view when you finish using the microscope.

Note: Usually the least magnification used in order to be able to see something in the water sample is 400x – so you need to use the 10x eyepiece and 40x objective lens.

 


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